Zucchini likes slightly acidic soil, so it is necessary to choose zucchini varieties that are disease-resistant, high-yield, high-quality, and have good appearance and shape. Suitable for production of strong seedlings, generally about 30 days old, with 3 to 4 true leaves, plant height about 10 cm, stem thickness about 0.5 cm, petiole length equal to leaf length, leaf color dark green, and cotyledons intact , Developed root system.

Before seed soaking and accelerating germination, the zucchini seeds should be dried in the sun for 2 to 3 hours, and then put into clear water, and the seeds floating on the water surface with insufficient grains should be removed and removed. Then soak the seeds with warm water at 55°C to 60°C for 10 minutes to further kill the pathogens on the seed epidermis. Then soak in warm water at about 25°C for 4 hours, remove it and control the moisture. Wrap it with damp gauze, and germinate at 28℃～32℃, and the seeds can be sown after they have broken their mouths and become white.

Regardless of whether it is using a seedbed to grow seedlings or a nutrient bowl, it is necessary to prepare a good nutrient soil first, which is the key to cultivating strong seedlings. Take three parts of fertile soil that has not been planted in melons within three or four years, one part of decomposed manure, add a small amount of plant ash and sawdust, mix well, sieve, and then make a border or a nutrient bowl. When ready, you can plant the seeds. After planting is completed, cover with two centimeters of soil. A small amount of bran mixed with organophosphorus pesticides can be sprinkled on the border surface to prevent underground pests. Then use a shed film to seal and moisturize until the seedlings emerge.

After emergence, temperature management should be strengthened. During the day, the temperature should be kept at about 20℃～25℃. If it exceeds 26℃, it should be ventilated and cooled. The temperature at night should be kept at 10℃～15℃, and the minimum temperature should not be lower than 6℃. At the same time, it is necessary to control the water. Only when there are obvious symptoms of lack of water can be watered. Do not water the big water. After watering, ventilate at the right time according to the weather and temperature, reduce the humidity, and prevent the occurrence of diseases.

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PCR Reagents

PCR Reagents

Real time PCR Kit for Monkeypox Virus is used for in vitro qualitative nucleic acid detection of Monkeypox Virus in Human serum, lesion exudate samples and scab specimens. Primer sets and FAM labeled probes are designed for specific detection of Monkeypox Virus, Human RNase P gene extracted concurrently with the test sample provides an internal control to validate nucleic extraction procedure and reagent integrity. Probe targeting human RNase P gene is labeled with VIC.


Intended Use
The test is intended for in vitro qualitative detection of monkeypox virus
(MPXV) antigen in human oropharyngeal swab, whole blood or skin lesion
materials, including lesion exudate, lesion roofs, lesion crusts.
The test is used for monkeypox virus antigen test of monkeypox suspected
populations. Positive result of the antigen test can be used for early triage and
rapid management of suspected populations, but it cannot be used as
diagnosis basis of monkeypox infection. Negative results do not rule out
monkeypox infection and should not be used as the sole basis for treatment or
patient management decisions. Further nucleic acid detection should be
carried out for suspected population whose antigen test result is positive or
negative.
The test is only for professional use, not suitable for family test. The test result
is only for clinical reference and it is recommended to conduct comprehensive
analysis of the disease condition in combination with clinical manifestations of
patients and other laboratory tests.
Test Principle
According to the gold immunochromatographic test principle, the
nitrocellulose membrane is coated with MPXV mAb 2 and goat anti-mouse IgG
antibody, the gold conjugate pad is labeled with MPXV mAb 1. When the
antigen is contained in the sample, the antigen binds with the corresponding
gold labeled monoclonal antibody to form a compound, moving forward under
the chromatography, then combines with the coated antibody in the test line
to form Au-MPXV mAb 1-antigen-MPXV mAb 2 complex to condense into a red
band (Test line, T), indicating a positive result. When the sample does not
contain antigen, complex cannot be formed in the test line, and no red band
appears, indicating a negative result.
No matter whether the sample contains antigen or not, the gold labeled
monoclonal antibody will combine with the coated goat anti-mouse IgG
antibody at the control line to form a Au-MPXV mAb 1-goat anti-mouse IgG
antibody complex and condense into a red band (Control line,C)


Pcr Reagents,Reagents Used In Pcr,Reagents Required For Pcr,Pcr Reagent Kit

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