1, slow cell growth or cell death
Under normal growth conditions, the cells are passaged once a day, the better the growth, the more the wall is attached.
Countermeasures :
1 medium formula is wrong;
2 The inoculation density is too low, below the 4th power of 10;
3 contaminated mycoplasma or other unknown bacteria, the status quo of mycoplasma is the appearance of small black spots; bacterial contamination is turbid; mold contamination is the appearance of white visible colonies; unknown bacterial pollution appears in the sand-like tiled background, the field of view is dark.
4 cell bottle is not cleaned
2, abnormal cell morphology
Under normal growth conditions, the cells are round, translucent, and grow in pairs, the better the growth, the more adherence, and less suspension.
Countermeasures :
1 medium formula is wrong;
2 The inoculation density is too low, below the 4th power of 10;
3 contaminated mycoplasma or other unknown bacteria, the status quo of mycoplasma is the appearance of small black spots; bacterial contamination is turbid; mold contamination is the appearance of white visible colonies; unknown bacterial pollution appears in the sand-like tiled background, the field of view is dark.
4 cell bottle is not cleaned
Frozen Sardine,Frozen Sardines Sardinefrozen,Frozen Sardine Fish,Sardine Whole Round Frozen
Zhoushan City Shuangying Aquatic Products Co., Ltd.  , https://www.shuangying-aquatic.com