Numerous studies have shown that changes in microRNA expression in the blood circulation are indicative of the stage of development and progression of the disease. At the same time, blood samples are easy to obtain in clinical, and stable and difficult to degrade, so it has broad research prospects for microRNA marker screening.

The challenge of using serum / plasma samples for microRNA research
• Serum plasma does not contain cells and has low microRNA content
• microRNAs are highly homologous, such as members of the hsa-let-7 family
• When hemolysis occurs in red blood cell rupture, substances that inhibit RT and PCR are released.
• Conventional internal reference (such as U6, etc.) is unstable in serum plasma and is not suitable as an internal reference control gene.

Accurate serum / plasma samples weapon microRNA
The miRCURY LNATM Universal RT microRNA PCR chip from Exiqon of Denmark combines the high-throughput advantages of the chip with the accurate and sensitive characteristics of PCR to achieve accurate and specific detection of microRNAs in various clinical samples such as serum plasma:
• Based on LNATM patented technology; unique Universal RT reaction
• Kangcheng microRNA research technology service for more than 8 years, customer SCI article more than 60 articles, average impact factor 5.5 points
Kang Cheng biological microRNA PCR chip technology services, click to view details>>

Human Serum/plasma panel (168 microRNA)
Designed for accurate detection of microRNA in serum plasma for direct microRNA marker screening
• Good repeatability: plasma samples, two independent experiments, correlation coefficient R 2 reached 0.96 (Figure 1)
• Strong specificity: distinguish between single base differential microRNAs; distinguish between mature microRNAs and precursors • High sensitivity: accurate detection of microRNA in serum plasma with detection limits as low as 5 copies

Low serum only 20 μ / L of plasma sample, 168 can achieve accurate specific detection of microRNA
• Unique Universal RT reaction: cDNA prepared by reverse transcription can be used as a template for all microRNA PCR reactions. • Low RNA requirements, especially suitable for serum/plasma detection, as low as 20 μL serum/plasma samples (Figure 2)
• Unified reverse transcription, simplifying experimental procedures, eliminating technical errors, and improving accuracy • Containing 168 microRNAs based on extensive literature data and extensive research results accumulated by Exiqon

Strict quality control system to ensure the reliability of experimental results
• Hemolysis determination: Use Nanodrop to test the hemolysis of the sample before the experiment to avoid substances that inhibit PCR • Evaluate the efficiency of the RT reaction: RT reaction set Spike-in
• Monitoring the PCR reaction process: Positive control in the PCR chip • Determination of amplification results: SYBR green PCR method can be used to determine amplification specificity according to the dissolution curve • Internal reference setting: Select stable and highly expressed microRNA in serum plasma (miR- 423-5P, etc. as internal parameters, calibration data



Strict quality control system to ensure the reliability of experimental results
• Hemolysis determination: Use Nanodrop to test the hemolysis of the sample before the experiment to avoid substances that inhibit PCR • Evaluate the efficiency of the RT reaction: RT reaction set Spike-in
• Monitoring the PCR reaction process: Positive control in the PCR chip • Determination of amplification results: SYBR green PCR method can be used to determine amplification specificity according to the dissolution curve • Internal reference setting: Select stable and highly expressed microRNA in serum plasma (miR- 423-5P, etc. as internal parameters, calibration data

Exiqon has microRNA screening and validation in the serum / plasma of thousands of patients with cancer, immune diseases, etc.




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