Ultra-thin sectioning technology is a common TEM sample preparation technology. It has a wide range of applications in the field of materials science. It is especially suitable for organic polymer materials and inorganic powder materials. It can be easily and conveniently obtained for nano-scale slicing for transmission electron microscopy. Observation; also has certain applications for metal materials and other inorganic materials. In addition, because this technology can also obtain the cross-section information of the sample very conveniently, it has certain applications in scanning electron microscopy and atomic force microscopy.
This lecture shows the experimental results of various types of samples, and elaborates on various types of sample preparation techniques, so that the audience can fully understand the application range of this technology, and at the same time, can learn the corresponding sample system. Kind of skill.
Excerpts from the wonderful questions and answers are as follows:
Q
Do you not dry the resin after cleaning the particles? Is it directly mixed with resin?
A: No, please pour off the supernatant organic solvent, leaving the precipitate mixed directly with the resin. The remaining small amount of organic solvent such as acetone will volatilize when cured at 60 degrees.
Q
Teacher, I am doing metal and ceramic composites. How do you cut such materials?
A: This type of sample does not need to be embedded, as long as the hardness is not very large, you can try. There are two suggestions. 1. The smaller the cut surface of the sample, the better. 2. If the metal is easily oxidized, try to use the dry cut method.
Q
Cut the surface of the aluminum alloy anodized film (3 microns), how to set the angle of the diamond knife?
A: Please use the diamond knife's recommendation Cl.Angle, usually written on the back of the diamond knife box, usually 6 degrees.
Q
How to embed 502 glue in inorganic particles?
A: 502 glue embedding samples, usually to deal with a few hundred micron samples, this direct fixture is too small, embedded and troublesome, you can use 502 speed dry glue to fix it to a large resin On the block, save the time of embedding.
Q
A kind of inorganic powder particles with a diameter of about several micrometers. After 812 embedding, the slices are all broken. How can I cut out a complete powder particle?
A: The sample is relatively brittle. The improvement direction is as follows: 1. Reduce the slicing speed, 2. Reduce the thickness of the slice, 3. Try using a 35-degree diamond knife (if you are using a 45-degree diamond knife), 4. Try Use an ultrasonic vibration diamond knife.
Q
Cutting inorganic and metal, it is easy to damage with a diamond knife. Is there any other tool?
A: No
Q
Is it necessary to remove the silicon substrate when slicing the coating material, or will the diamond knife be damaged?
A: No, if the operation method is right, the damage of the diamond knife to the diamond is controllable. In my personal experience, many times, the damage to the diamond knife is not caused by slicing, but improper operation when the knife is used. A very important detail in cutting such materials to protect diamond knives is that when knives, do not return the knife.
Q
We are doing biological samples. There are some impurities in the in vitro cultured cells. When you cut them, you will use a knife. According to your explanation today, diamond knives should be able to overcut those impurities. I would like to ask if the inorganic materials are prepared by ultra-cut samples. Need to soften?
A: No, because the sample after softening is not the sample being studied.
Q
Is it possible to use it in life sciences? Above the plants?
A: Yes. This technology has a wide range of uses in the life sciences.
Q
The principle of this ultra-thin section is achieved with an ultrasonic diamond slicing knife?
A: No.
Q
Cut the surface of the aluminum alloy anodized film (3 microns), how to set the angle of the diamond knife? Then this question, in the process of repairing the glass knife, is the angle still 6 degrees?
A: 6 degrees.
Q
How to cut larger bones for AFM observation?
A: 1. If the sample is large, a wide diamond knife can be used. The largest diamond knife width is 8mm. 2. If it is for AFM observation, there is a fixture for AFM.
Q
The metal-based composite material formed by dispersing the nano-alumina particles in the aluminum alloy is a bulk material, can it be cut?
A: This type of sample does not need to be embedded, as long as the hardness is not very large, you can try. There are two suggestions. 1. The smaller the cut surface of the sample, the better. 2. If the metal is easily oxidized, try to use the dry cut method.
Q
After cutting the rubber, the sample becomes normal temperature, and the shape changes greatly?
A: I don't know. However, many of the existing researches do this, indicating that their topographical changes are within the scope of acceptance.
Q
Can the resin be mixed with acetone?
A: 812 can be mixed with acetone for gradient infiltration. For other resins, please refer to the resin instructions.
Leica related equipment
Leica EM UC7 ultra-thin slicer
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