The NF-κB/Rel transcription factor family contains several structurally related proteins (p65/p105, p52/p100, RelA (p65), c-Rel/NF-κB) to form homologous and/or heterodimers. Members of the transcription factor family regulate the expression of more than 150 genes of interest, including inflammatory cytokines, chemokines, immune receptors, cell adhesion molecules, and the like. Because of this, NF-κB is considered to be “a central regulator of immune response”. As a dimer, these transcription factors bind to DNA sequences (these binding sites are referred to as "κB sites"), thereby regulating the expression of the gene of interest. In most cells, the NF-κB/Rel transcription complex is present in the cytosol in an unactivated form, binding to the inhibitor IκB. When the cells are stimulated, some phosphorylation and ubiquitination reactions lead to degradation of IκB, and NF-κB transcription factors enter the nucleus.

The role of NF-κB transcription factor in human inflammatory response and other diseases has attracted more and more researchers' attention, and the detection of NF-κB subunit activity in cells is essential for NF-κB research. One step!

How to safely, conveniently and effectively detect the activity of NF-κB subunits in cells?

Cayman Chemical gave the answer: NF-κB (p65) Transcription Factor Assay Kit (item number) and NF-κB (human p50) Transcription Factor Assay Kit (item number). In the previous sample processing, the cayman Chemical Cell Nuclear Extraction Kit (Nuclear Extraction Kit, item number) can also be used.

NF-κB (p65) / NF-κB (p50) transcription factor analysis kit principle:
The microplate is pre-coated with dsDNA oligonucleotide containing NF-κB binding site, added to nuclear extract, NF-κB protein is bound to dsDNA, washed to remove unbound component, and NF-κB specific antibody is added successively. And the HRP-labeled secondary antibody. After the enzyme substrate was added, the OD450 value was read by enzymatic reaction to quantify the activity of NF-κB in the sample.
Figure 2: Principles of NF-κB (p65) / NF-κB (p50) transcription factor assay kit
NF-κB (p65) / NF-κB (p50) transcription factor assay kit features:
●Replaces the traditional gel migration or electrophoretic mobility experiment (EMSA), which enables rapid high-throughput detection of large numbers of samples based on the ELISA reaction principle.
Detecting NF-κB activity at the level of DNA-protein interaction and detecting NF-κB in the cell that is truly activated
●The kit contains NF-κB (p65)/NF-κB (p50) transcription factor positive control
NF-κB (p65) transcription factor assay kit detects NF-κB (p65) in human, mouse and rat, does not cross-react with NF-κB (p50); NF-κB (p50) transcription factor analysis The kit detects human NF-κB (p50) and does not cross-react with NF-κB (p65)
Figure 3: NF-κB (p50) / NF-κB (p65) transcription factor analysis kit results show

Micro Laser Distance Sensor

New product of U85 micro laser distance sensors use highly focused class 2 laser to detect objects or measure distances, and can return a measured value via varieties intface( serial, usb, rs232, rs485, bluetooth etc.). The electronic distance sensor is a very small Laser Distance Sensor, but high resolution up to 1mm and long distance measuring sensor - teachable measuring range of up to 30m. Extremely accurate distance sensing sensors, errors down to ± 1mm. And the mini sensors and measurements support continuous measurement function, great for compact solutions(eg: robots) with the smallest Laser Distance Sensor of the world!

U85 Micro Laser Distance Sensor & MCU control board connection

Parameters of U85:


Accuracy

±1 mm (0.04 inch)

Measuring Unit

mm

Measuring Range (without Reflection)

0.03-20m/0.03-30m

Measuring Time

0.1~3 seconds

Laser Class

Class II

Laser Type

620nm-690nm, <1mW

Size

41*17*7mm (±1 mm)

Weight

About 4g

Voltage

DC2.0~3.3V

Electrical Level

TTL/CMOS

Certifications

CE, FCC, RoHS, FDA

Operating Temperature

0-40 ℃ (32-104 ℉ )

Storage Temperature

-25~60 ℃ (-13~140 ℉)

Mini Laser Distance Sensor,Optical Laser Distance Sensor,Smallest Laser Range Sonsor,Laser Measuring Sensor

Chengdu JRT Meter Technology Co., Ltd , https://www.jrt-measure.com