Regulate in vitro follicular development to find new targets
December 3, 2018 Source: Health News Author: Yang Dong Ping
Window._bd_share_config={ "common":{ "bdSnsKey":{ },"bdText":"","bdMini":"2","bdMiniList":false,"bdPic":"","bdStyle":" 0","bdSize":"16"},"share":{ }};with(document)0[(getElementsByTagName('head')[0]||body).appendChild(createElement('script')) .src='http://bdimg.share.baidu.com/static/api/js/share.js?v=89860593.js?cdnversion='+~(-new Date()/36e5)];On November 21st, the team of the third hospital of Peking University, Qiao Jie, published a paper entitled "Troublemic regulation of human follicular development and interaction between oocytes and granulosa cells" in the "Molecular Cell" publication of Cell magazine. Analyze the transcriptome dynamic panorama and the regulation of gene expression regulation of oocyte-granule cell interaction during human follicle development, which is helpful to establish an objective standard for evaluating follicular development, to accurately predict ovarian reserve, monitor fertility level, and screen Markers of oocyte maturation provide clues to provide potential targets for improving in vitro follicular development and oocyte maturation.
In this study, we revealed the gene expression characteristics of oocytes and granulosa cells from the level of single follicles, and mapped the dynamics of the transcriptome during human follicular development and analyzed the interaction of oocyte-granule cells, while on mice and human eggs. Comparative analysis of maternal transcriptome data revealed conserved and species-specific genes involved in the regulation of follicular development.
There are five main findings in the study: First, the overall gene expression patterns of oocytes and granulosa cells are significantly different. With the development of follicles, the expression level of meiosis-related genes in oocytes was up-regulated, while the expression level of mitotic-related genes in granulosa cells was up-regulated. Second, during the development of follicles, oocytes and granulosa cells have stage-specific genes. Third, the genes related to Insulin, GnRH, Neurotrophin and mTOR signaling pathway were significantly up-regulated in oocytes and granulosa cells of primary follicles, which may be involved in the activation regulation of primordial follicles to primary follicles. Fourth, granulosa cells may regulate oocyte development through the KITLG-KIT signaling pathway, and oocytes may regulate granulosa cell proliferation and differentiation through the NOTCH signaling pathway. The expression patterns of human and mouse oocyte developmental genes are different. Among them, POUF5, GJC1, TEAD2 and BMP15 have different expression patterns, and HAS3, HTR5A, SNPH and NRAP have similar expression patterns.
Prof. Qiao Jie, Associate Researcher Yan Jie and Researcher Yan Liying from the Third Hospital of Peking University are the authors of the paper. Dr. Zhang Yiyao, Peking University Third Hospital, Yan Zhiqiang, Ph.D. student of Peking University Tsinghua Life Science Joint Center, and Yan Qingyuan, a graduate student of Peking University Third Hospital, are the first authors.
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