Spring blossoms, everything recovers. When we are immersed in this beautiful spring, when we feel contented, the lab's cell culture experts may have to pay attention, and the bacteria, fungi and mycoplasma that harm cells are also active. How do we prevent prevention during cell culture? Xiaobian summed up some, I hope to help the lab's partners!
Prevention is the essential idea in the cell culture process! March and April do not prevent, May June is sad!
First, good aseptic operation
1. All relevant materials and reagent bottles for cell culture should be sterilized completely.
2. The preparation of cell culture fluid and related reagents should be strictly aseptically operated.
3. Prepare the required experimental equipment and articles before the experiment to avoid going back and forth into the cell culture area.
4. Do not place too many supplies or overlap in the clean bench, otherwise it will block UV irradiation and reduce the sterilization effect.
4. Pipette the pipette, waste liquid cylinder, test tube, etc. with a 75% alcohol cotton ball before the culture, and then irradiate the UV in the clean room for 15 minutes.
6. Do not touch the mouth of the cell culture bottle when inhaling or aspirating liquid.
7. The culture solution should be stored in parallel and stored at 4 ° C.
8. Each cell should use a separate medium
9. Wear masks and sterile gloves for cell culture to reduce conversations.
10. After each experiment, clean the table top with 75% alcohol and irradiate for 15 minutes with UV light.
Second, the rational use of antibiotics
Do not overuse antibiotics. Abuse of antibiotics often leads to the development of resistant bacteria, keeping in mind that antibiotics cannot replace aseptic operations.
Third, establish a cell bank
Fourth, good rules and regulations
1. Only relevant experimental personnel can enter the cell
2. Cells must be separated from other experimental areas
3. Cell culture areas should avoid the use of pools to avoid microbial contamination
Fifth, keep the cells clean
1. Regularly clean and disinfect the floor and countertops
2. The CO 2 incubator should be disinfected regularly (2-3 months). All spilled or spilled droplets should be wiped and disinfected immediately. The water tray in the incubator should be thoroughly disinfected and replaced with sterile water.
3. Clean up waste and susceptible materials in time
Certain related product reagents help us prevent early and find out early!
The PromoCell PCR Mycoplasma Detection Kit can be used to periodically detect mycoplasma/bacterial contamination in cell culture for early detection and prevention. Source culture biological mycoplasma removal reagents can be used to solve the problem of cell contamination or degraded state caused by mycoplasma contamination, and it also has a certain scavenging effect on common Gram-negative and positive bacteria. The specific item number list is as follows:
name | Item number | |
PCR Mycoplasma Test Kit I/C | PK-CA91-1024 PK-CA91-1048 PK-CA91-1096 | Click to query |
PCR Mycoplasma Test Kit I/RT | PK-CA91-3025A PK-CA91-3025B | Click to query |
PCR Mycoplasma Test Kit II | PK-CA91-20-700-20 | Click to query |
name | Item number | |
Mycoplasma preventive reagent | S280, S280B | Click to query |
Mycoplasma removal reagent | S270, S270B | Click to query |
LED3 and LED5 Operating Theatre Lights
Shadowless Lamp,Led Operating Lamp,Led Operation Lamp,Led3 And Led5 Operating Theatre Lights
NINGBO MEDICAL EQUIPMENT CO.,LTD , https://www.techartmeds.com