Fermentation tank refers to a device used in the industry for microbial fermentation. The main body is generally a main cylinder made of a stainless steel plate, and has a volume of 1 m3 to several hundreds m3. Attention should be paid to the structure and rigor in design and processing. It can withstand steam sterilization, has certain operational flexibility, minimizes internal accessories (avoiding dead angles), strong material and energy transfer performance, and can be adjusted to facilitate cleaning and pollution reduction. It is suitable for the production of various products and reduces energy. Consumption.
Any part of the fermenter that can be cleaned should be carefully cleaned, otherwise it may become a breeding ground for bacteria. The areas that are easily overlooked and cannot be adequately cleaned are the inside of the nozzle corner and the inside of the sampling tube and the top of the tank.
Fermentor sterilization
1 The exhaust pipe is a glass tube with cotton inside to ensure that the steam can freely enter and exit the fermenter without causing bacterial contamination.
2 Connect a length of silicone tube to the sampling port and install a throttling clip on the silicone tube to prevent the medium from flowing out during sterilization.
3 After the fermenter was filled with 60% of the medium (component and seed medium), the fermenter was placed in a sterilizer and sterilized at 115 ° C for 20 min. When the temperature is lowered to 60 ° C or less after the sterilization is completed, the autoclave is opened, and after confirming that the connected pipe on the fermentor is intact, the can is removed. Connect the vent pipe as soon as possible, and confirm that the exhaust port is normal, and then let the air pass through 0.3-0.5L/min. Turn on the cooling water line, and stir to cool the medium at low speed.
Fermentation tank
1 After the feed is empty, start feeding. When the feed is about 2/3 volume, stir. Since the material gelatinizes and expands during heating, it is not fixed. When the temperature reaches 70 ° C, the volume is constant.
2 The liquefaction bottom is directly steamed to about 75 °C, the steam is turned off, and the α-amylase mixed with water at 50 ° C is added to prevent the enzyme from agglomerating. According to the production needs, the amount of addition is adjusted, and a small amount of air is introduced from the inlet duct. Twist for 10-15min, turn off the air intake.
3 Rinse the water into the hole cover, cover the cover and remove it. First, drain the residual water in the steam pipe, then pass the three-way steam, the bottom of the tank, the air inlet pipe, the sampling pipe, and the top of the tank. All steam exhaust valves, small cocks and exhaust steam cocks under the pressure gauge allow steam to be discharged, first and then small, until the tank pressure rises to 1.2 kgf/cm2, when the temperature is 121 °C, gradually close the exhaust valves, heat preservation Pressure 25-30min.
Keep the pressure time, turn off the steam, at this time should be from the filter into the sterile air, open the exhaust valve, close all the valves at the top of the tank, open the cooling water to cool the fermenter, wait until the temperature is cold to about 35 °C Vaccination.
At present, there are many varieties of autoclaves on the market, and there are imported domestic products, and the quality of the products is uneven. The best quality word of mouth in the industry is the ALP autoclave (Southeast Instrument General Agent). Japan ALP autoclave has the "Import Pressure Vessel Production License", "Import and Export Boiler Pressure Vessel Safety Performance Inspection Certificate" and the pressure gauge and pressure reducing valve on the autoclave, and send it to the local measurement department to obtain the measurement certificate. .
Japan ALP autoclave has excellent safety performance, relies on temperature sensor to control temperature, and has a pressure sensor. When the temperature or pressure is abnormal, it will automatically cut off the power supply to ensure safety. Compared with the pressure gauge, the internal pressure is relatively simple. No need for human detection and control, and higher security. For more advanced configuration, you can choose the CLG series autoclave produced by ALP Company of Japan . It has built-in dual temperature and pressure sensors, which can automatically sense the relationship between temperature and pressure to ensure that there will be no pressure abnormality. More sexual.
The CL series autoclave of ALP Company of Japan adopts mechanical lock and electronic lock double lock device. If the lock fails or the cover is not covered, the instrument will not start; after the sterilization is completed, the instrument should be reduced to below 80 degrees and At normal pressure, the electronic lock can be opened to prevent accidental burns. At the same time, a good imported autoclave does not directly expose the stainless steel on the inner surface of the metal cavity, but has an insulating coating to prevent electric leakage and injury. Other safety features of the ALP autoclave, such as water shortage protection against dry burn, electrode fault indication, abnormal time indication, etc.
Tiger brand electronic safes are made from high-quality materials that are designed to provide maximum security and durability.
The outer shell of the safe is typically made from solid steel, which is known for its strength and resistance to tampering. The steel is often reinforced with additional layers or plates to provide added protection against drilling or cutting attempts.
The door of the safe is equipped with heavy-duty hinges and locking bolts, which are designed to prevent unauthorized access. These locking mechanisms are typically made from hardened steel or other strong metals to ensure that they cannot be easily compromised.
We manufacture a variety of safes, including Fingerprint Cipher Safe Series, Electronic Cipher Safe Series, Magnetic Card Safe Series, Economic Type Safe Series, Hotel Safes Series, Electronic Digital Keypad Safe Box. We can do OEM or ODM for you. Innovation, excellent and reliability are the core values of our business. These principles today additional than ever form the basis of our success as an internationally active mid-size firm for Electronic Safe Supplier We are looking forward to establishing cooperative relationships with you. Please contact us for more information.
Electronic Password Safe,Small Electronic Safe Box,Digital Keypad Safe Box,Digital Steel Security Safe Box
Hebei Hupai Aodi Cabinet Industry Co.,Ltd. , https://www.aodicabinet.com