GFP (Green Fluorescent Protein) was discovered by Xia Cun Xiu et al. in 1962 in a jellyfish of the scientific name Aequorea victoria. The protein produced by its gene emits green fluorescence when excited by light in the blue wavelength range. The GFP tag can be located at the C-terminus or N-terminus of the protein. This system has been widely used in various cell types, including bacteria, yeast and mammalian cells, and the corresponding GFP-tagged antibodies are also widely used. In general, GFP antibodies can not only detect 
GFP or an appropriate mutant thereof can also detect expression, intracellular localization of fusion protein expressed by GFP or its appropriate mutant, and purification, qualitative or quantitative detection of GFP fusion-expressed protein and the like.
How to choose the right and most cost-effective GFP antibody?
When choosing a label antibody, we mainly rely on the needs of our own experimental application. Factors to be considered here include the type of detection of the GFP antibody, the cloning of the GFP antibody, and the source of preparation. For example, can the GFP antibody be used for the detection of immunofluorescence IF? Can it be used in Western Bloting and immunoprecipitation IP experiments? Do you need a more specific monoclonal GFP-tagged antibody? In general, the more types of applications, the greater the choice in use, and the monoclonal antibodies from mouse sources generally have better specificity, stability and potency than polyclonal antibodies. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use.
I bought a domestic GFP antibody before, the price is cheap, the effect is also average; later bought Abbkine GFP monoclonal antibody, bought a, although the price is more expensive, but the price is more than an order of magnitude higher, the key is to There are many types of experiments, except for WB, I also did IP experiments. In the end, the price/performance ratio is better than that of the domestic one.
——  Customer, First Affiliated Hospital of Zhejiang University School of Medicine
| Product name and description | Brand | Item number | product manual |
| Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | Abbkine | A02020 | Details |
| Anti-GFP Tag Rabbit Polyclonal Antibody | Abbkine | A02025 | Details |
Why choose Abbkine's GFP monoclonal antibody (Cat. No. A02020)?
Currently, GFP antibodies available on the market are available in domestic packaging or in origin; there are also many imported brands that are imported from GST, such as Abcam, Santa Cruz, Cell Signaling (CST), and Millipore. However, because it is not a specialized label and internal reference antibody supplier, it is not the best choice for cost performance. For Abcam, for example, a 100μl GFP antibody is quoted at ¥4418; a CST 100μl GST antibody is quoted at ¥2997.
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The domestically packaged GFP antibody is insufficient in quality stability, and its valence is also lost after being packaged. However, some GFP antibodies originating in domestic laboratories have better detection activity, but there are gaps in application types, and it is difficult to control the stability between batches, and only a small amount of domestically produced antibodies are also available in China. Polyclonal antibody. Here, we recommend the American GFP antibody Anti-GFP Tag Monoclonal Antibody (3D3) (Cat. No. A02020). The GFP antibody is a monoclonal antibody and the clone number is 3D3. Its antigen is the N-terminal polypeptide of KLH-conjugated jellyfish GFP protein. Abbkine's GFP-tagged antibody (Cat. #A02020 or A02025) is 65% lower than Abcam and only half the price of CST. Both monoclonal and polyclonal antibodies can be used in Western Bloting experiments to analyze the expression levels of GFP fusion proteins.
| Product name and description | Brand | Item number | product manual |
| Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | Abbkine | A02020 | Details |
| Anti-GFP Tag Rabbit Polyclonal Antibody | Abbkine | A02025 | Details |
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| Click image to view GFP antibody more details |
A high titer antibody not only affects the sensitivity of the experimental results, but also means a higher price/performance ratio. Abbkine's GFP-tag antibody In the conventional WB experiment, the recommended initial dilution ratio is 1:1000. Actually, in the application of Abbkine, there is a dilution ratio of 1:5000 or higher, which needs to be based on the specific experimental conditions of the customer. optimize.
Abbkine also offers 1ml large packaging and larger custom-made GFP-tagged antibodies, making it ideal for routine GFP immunoassay experiments. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact your local agent Ai Meijie Technology Co., Ltd. directly.
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| Product name and description | Brand | Item number | product manual |
| Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | Abbkine | A02020 | Details |
| Anti-GFP Tag Rabbit Polyclonal Antibody | Abbkine | A02025 | Details |
The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its regional agent in China, and will provide customers with the most cost-effective routine immunological reagents, such as A tag antibody, an internal reference antibody, a conventional primary antibody, and a full range of secondary antibodies, antibody detection substrates, and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to 801/802/803 or 027-84454412/4876 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.
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