Beijing Zhongwei Weikang Safety Quantitative Check Card iCheck® Salbutamol Quantitative Check Card (Meat Sample)

1. Summary

A beta - agonist is an adrenal receptor agonist, a neurostimulant, chemically similar to a derivative of phenylethanolamine. Such drugs are used clinically to treat diseases such as bronchial asthma, chronic bronchitis and emphysema. Because this medicine can increase lean meat rate, reduce fat deposition and promote animal growth, it is illegally used by some animal husbandry enterprises as a culture promoter. After humans eat the animal products produced by the animals supplemented with clenbuterol as an additive, muscle tremors, palpitations, nervousness, headaches, muscle pain, usually occur due to the poisoning of clenbuterol remaining in the animal products. Symptoms such as dizziness, nausea, vomiting, fever, and tremors are extremely harmful to the health of consumers. China Ministry of Agriculture Ministry of Agriculture No. 235 announced that Clenbuterol and its salts and esters should not be detected in all food animals. The iCheck® salbutamol quantification card is capable of quantitatively detecting tissue samples in the 0.-10.0 ppb range.

2. Salbutamol quantitative quick test card test kit

1) iCheck® Salbutamol Quick Check Card ( 25 )

2) SAL micropores ( 25 )

3) Dilution buffer ( 5 mL)

4) 1.5mL centrifuge tubes ( 25 )

5) 1mL tips (25)

6) 200μL tips ( 50 )

7) SAL QR Code ( 1 )

8) Operating instructions

3. Equipment required for testing

1) 200μL, 1000 μL micropipettes ( 1 each)

2) iCheck® Quantitative Tester ( with zero calibration film )

3) Centrifuge ( 7000 rev / min, or higher)

4) Timer

4. Preparation before testing and instrument calibration

1 ) Restore the quick check card, buffer and sample to be tested to room temperature (25 °C ± 2 °C ) for 30 minutes before use.

2 ) Equipment zero calibration: Insert the zero calibration piece into the quantitative quick test chamber and click “ Zero Correction ” .

3 ) Equipment calibration: Insert SAL QR Code into the quantitative quick checker card slot and automatically detect “ calibration ” .

4 ) According to the number of samples, open the package, take out the quick check card and SAL micro-holes, and place the SAL micro-holes on the micro-hole rack.

5. Sample processing and testing

1) Take 5.0g homogenized meat samples in 50mL centrifuge tubes (if not detected immediately, the sample can be kept refrigerated), the pot was heated at 80-90 deg.] C water bath for 20 minutes, 4000 rpm / min, centrifuged for 10 minutes, take a clear The upper liquid is the sample to be inspected.

2) Take 50 μL of the dilution buffer in the microwell, add 100 μL of the upper clarified sample, mix it with a phase shifter for 5 times, and add 100 μL to the sample hole of the quick check card.

3 ) After reacting at 37 °C (± 2 °C) for 10 minutes, immediately put the quick check card into the quantitative quick tester and click the “ analyze ” reading, which is the actual concentration of the sample.

6. Result determination

1) Detection range: 0-10.0ppb, limit of quantitation 1.0ppb.

2 ) This test card is a quantitative quick check card. If there is no red line on the control line ( C ), it indicates that the quick check card is invalid.

3 ) The measured value is 10.0 ppb . Dilute the sample with water. Finally, quantify the reading in the rapid tester by the dilution factor, which is the actual concentration of the sample.

7. Precautions

1) The quick check card is used once during the warranty period. Store the refrigerator at 2-8 °C until it is equilibrated to room temperature (25 °C ± 2 °C) before use.

2) Beware of the moisture in the quick check card, the aluminum foil packaging bag is damaged or the quick check card is damp, the test card should not be used.

3) Try not to touch the white film in the center of the quick check card.

4) Avoid direct sunlight during all incubations.

5) The small bag in the aluminum foil packaging bag is a desiccant and should not be taken into account.

6) Exceeding the shelf life cannot be used.