product description
| Â English name | Â Human MYO (Myoglobin) CLIA Kit | ||
|  Chinese name  |  Human Myoglobin (MYO) Chemiluminescence Immunoassay Kit  | ||
| Â Item number | Â E-CL-H0134c | Â species | Â Human / person |
| Â specification | Â 96T/Kit (8*12 strips) 48T/Kit (8*6 strips) | ||
|  Detection method  |  Double antibody sandwich method  | ||
| Â examination range | Â 3.125~200ng/mL | Â Sensitivity | Â 1.875ng/mL |
Fundamental
This kit uses a double antibody sandwich method. The anti-Human MYO antibody was coated on the plate, and the MYO in the specimen or standard was bound to the coated antibody, and the free component was washed away. Biotinylated anti-Human MYO antibody and horseradish peroxidase-labeled avidin were sequentially added. The anti-Human MYO antibody binds to Human MYO bound to the coated antibody, and biotin binds to avidin to form an immune complex, and the free component is washed away. The luminescent substrate mixture was added, the luminescent substrate was fluoresced under the catalysis of horseradish peroxidase, and the chemiluminescence value (CL) was measured by a chemiluminescence immunoassay analyzer. The MYO concentration was positively correlated with the chemiluminescence value. The standard curve is used to determine the concentration of MYO in the specimen.
Steps
1. Add 100 μL of standard or sample to each well and incubate at 37 ° C for 90 minutes.
2. Pour the liquid in the well, pat dry, add 100 μL of biotinylated antibody working solution, incubate at 37 ° C for 60 minutes.
3. Wash 3 times
4. Add 100 μL of enzyme conjugate working solution and incubate at 37 ° C for 30 minutes.
5. Wash 5 times
6. Add 100 μL of substrate solution and incubate for 5 minutes at 37 °C
7. Readings
8. Calculation of results
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