Strengthening sow rearing management
Sows should be fed more roughage during the rest period, mid-pregnancy and late lactation, and be fed with green feed properly. Sows are supplied with sufficient protein, minerals, and vitamins during the early stages of pregnancy, late pregnancy, and early breastfeeding. At the same time, it is necessary to provide the sow with a relatively stable living environment, and to minimize the stress response caused by transfer, drive, and the like.
Do a good job of giving birth to a sow
The sows are cleaned 5 to 10 days before delivery and sprayed with 10% to 20% fresh lime water. The sows are sterilized with 2% to 5% of the pregnant women's abdomen, breasts, and pus. After the sow has given birth, the mucus in the nose of the piglet must be removed in time to tear off the membranes. For juvenile piglets, it can be used to pat the chest, lift the hind legs or use alcohol to punish the nose. For the difficult-to-produce sows to do a good job midwifery, so that the sows will have a smooth delivery. In addition, heat preservation is especially important for newborn piglets. When the piglets are born, the temperature of the tillers must be maintained at 26°C~32°C.
Sows have to breed during lactation
Piglets are weaned at 35-40 days of age. In general, aphrodisiacs are given to sows 3 to 5 days after weaning. After sows are estrus, they must be bred immediately to make them pregnant during lactation to increase litter size.
The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination.
Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.
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